• The advantages of CP could be even more profound for

  2018-10-22

  

  The advantages of CP could be even more profound for manufacturing cell therapies. In a typical cell therapy workflow, hPSCs are expanded and banked in a GMP facility before undergoing expensive and time-consuming tests to validate the cell bank. The conversion of this PSC bank into a therapeuticall

• Genomic changes at the chromosomal or single nucleotide leve

  2018-10-20

  

  Genomic changes at the chromosomal or single nucleotide level likely contribute to the variability of the pluripotent states and differentiation potential. These changes can be acquired and accumulated during cell culture because of suboptimal growth media, inappropriate extracellular matrices, and

• As METH impairs self renewal capacity we also explored the

  2018-10-20

  

  As METH impairs self-renewal capacity, we also explored the influence of METH in DG stem cell-fate division, a suitable method to characterize the phenotype of cells that are derived from the division of one DG stem cell. Indeed, a stem cell can undergo symmetric cell division towards self-renewal,

• Several lines of evidence suggest that mitochondrial insuffi

  2018-10-20

  

  Several lines of evidence suggest that mitochondrial insufficiency and failure to leave glycolysis (Warburg metabolism) are the fundamental limits to normal differentiation after FGF4 removal. These lines of evidence also pinpoint the two lineages at the labyrinthine surface as the highest energy- a

• We next aimed to test the differentiation capabilities of

  2018-10-20

  

  We next aimed to test the differentiation capabilities of the EZ spheres. We found that at any passage for all cell lines regardless of cryopreservation, EZ spheres can be placed in specific neural induction media for terminal neuronal or glial cell differentiation using protocols adapted from those

• br Acknowledgments This study was funded by a grant provided

  2018-10-20

  

  Acknowledgments This study was funded by a grant provided by the Royan Institute. We thank Mr. Ehsan Janzamin for his unlimited assistance in the Flow Cytometry Laboratory, Dr. Abass Piryaei and Leila Sattarian in the Histology Laboratory, Mr. Chehrazi for statistical analysis, and Miss Saloomeh

• The following is the supplementary data related

  2018-10-20

  

  The following is the supplementary data related to this article. Author contribution Acknowledgments Introduction Endothelial progenitor alzheimer\'s disease (EPCs) circulate in peripheral blood and migrate to a site for vascular formation and repair (Asahara et al., 1997). The pro-angiog

• br Introduction Stem cells play a crucial role in maintainin

  2018-10-20

  

  Introduction Stem wst-1 play a crucial role in maintaining tissue homeostasis. In this context, interplay between stem cells and their microenvironment, also known as the niche, is necessary for the maintenance of stem cell phenotypic state (Scadden, 2014). In general, distinct stem cell populat

• The karyotype of the MPSII iPSC line

  2018-10-20

  

  The karyotype of the MPSII-2.5 iPSC line was determined by Giemsa-banding, proving normal colorimetric assay 46, XY karyotype, without any detectable abnormalities (Fig. 1B). Materials and methods Acknowledgement We would like to thank the Department of Pediatrics, University of Pécs (Hungar

• br Results br Discussion The availability of Lgr as a

  2018-10-20

  

  Results Discussion The availability of Lgr5 as a specific marker for stem STF-62247 in the intestine and other tissues has allowed the unequivocal identification of intestinal stem cells, as well as molecular profiling (Muñoz et al., 2012), the establishment of culture methods (Sato et al.,

• We propose that a quantitative

  2018-10-20

  

  We propose that a quantitative marker for tracking cardiac myocyte maturation resides in the developmentally controlled and irreversible (excepting targeted reprogramming) genetic switch in the sarcomeric TNNI gene. Owing to strict developmental control in all mammalian hearts, including humans, the

• purchase Madecassic acid In our previous studies Choi et al

  2018-10-20

  

  In our previous studies (Choi et al., 2012; Vodyanik et al., 2006, 2010), we identified distinct stages of hematoendothelial development following hPSC differentiation in coculture with OP9 (Figure 1). Plating hPSCs onto OP9 stromal purchase Madecassic acid induces the formation of primitive streak

• Expression of Sox alone was sufficient to induce neurogenesi

  2018-10-20

  

  Expression of Sox2 alone was sufficient to induce neurogenesis without requirement for coexpression of a proneural transcription factor. This is surprising, as Sox2 function in neural stem cells is to promote self-renewal rather than neuronal differentiation (Graham et al., 2003; Pevny and Nicolis,

• MSCs can express several WNT ligands Cho et al

  2018-10-20

  

  MSCs can express several WNT ligands (Cho et al., 2006; Etheridge et al., 2004). This endogenous WNT signaling may play a negligible role in MSC expansion, because we found no significant effects on cell proliferation or subsequent differentiation when endogenous WNT proteins were prevented. However

• Many of the same pathways that trigger obesity

  2018-10-20

  

  Many of the same pathways that trigger obesity-associated beta cell dysfunction in patients with type 2 diabetes may also contribute to graft failure following islet transplantation (Potter et al., 2014). Therefore, a potential caveat in replacing dysfunctional beta clofibrate with healthy insulin-

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