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Two postoperative complications occurred in
2018-10-22
Two postoperative complications occurred in 14 patients. Patient 5 suffered from wound dehiscence after radiotherapy but healed completely through secondary intension. The propeller perforator flap in Patient 9 exhibited a total loss because of venous congestion, and a salvage full-thickness skin gr
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In recent years researches on
2018-10-22
In recent years, researches on anthocyanins have attracted more and more attentions, especially their isolation and purification [13–15]. In contrast, there have been few studies focusing on chemical structures and antioxidant activity of anthocyanins in edible rose. Therefore, the aim of this study
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br Materials and methods br Results and
2018-10-22
Materials and methods Results and discussion Conclusion In summary, some members of the fecal bacteria utilized garlic fructans to grow resulting in a decrease in the pH of the cultures in vitro. Bifidobacteria were stimulated while Clostridia were inhibited by the garlic fructans. Essentia
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br Diseases and disorders caused by alterations in
2018-10-22
Diseases and disorders caused by alterations in the human gut microbiota It is evident that prenatal maternal exposure influences postnatal microbial colonization [3] and this plays pivotal roles in gut-associated lymphoid tissue (GALT) development [7], specific aspects of immune system developme
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Tr vo a standardized phytopharmaceutical supplement manufact
2018-10-22
Trévo™, a standardized phytopharmaceutical supplement manufactured in the US is presented as a homogenous mixture of plant-based extracts, water and lipid soluble vitamins, mineral nutrients and other components (Supplementary file). It is touted by users for health-promoting effects and as a remedy
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GFP expressing Rhesus nhpESC lines Table
2018-10-22
GFP-expressing Rhesus nhpESC lines (Table 1) used in the aggregation chimera assay produced expanded mouse blastocysts with GFP-expressing nhpESC lines 2706 and 3006 associated with the blastocyst inner cell mass (ICM) cells. We typically combined mouse zona pellucida-free 2-to-8-cell stage embryos
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Previous experiments with mouse chimaeras and X inactivation
2018-10-22
Previous experiments with mouse chimaeras and X-inactivation mosaics (Collinson et al., 2002, 2004; Mort et al., 2009) suggested that the numbers of active LESC clones decline with age in wild-type mice and are reduced in Pax6+/− heterozygotes. Although this would imply that LESC function is deficie
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The immunophenotype of all three populations was comprehensi
2018-10-22
The immunophenotype of all three populations was comprehensively compared using a panel of 81 PE-conjugated tetracycline hcl (Supplementary Table 1). A heat map showed extensive congruence in the percentage of cells positive for a given CD epitope between the three populations, although hierarchical
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We aimed here to elucidate whether the effect
2018-10-22
We aimed here to elucidate whether the effect of taurine increasing proliferation occurs on stem cells as well as on neural progenitor cells. Neurospheres are formed by caspofungin of neural progenitor cells around one or several stem cells, but may also be formed by clusters of progenitor cells on
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To define the transcriptome of the ExE
2018-10-22
To define the transcriptome of the ExE lineage in the present study, we analyzed primitive endoderm and trophectoderm markers using qRT-PCR. Interestingly, we found that markers of the primitive endoderm, such as DDAH2, LAMB1, DAB2, AFP and NR2F1 (COUP-TF1), and markers of the trophectoderm, such as
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The advantages of CP could be even more profound for
2018-10-22
The advantages of CP could be even more profound for manufacturing cell therapies. In a typical cell therapy workflow, hPSCs are expanded and banked in a GMP facility before undergoing expensive and time-consuming tests to validate the cell bank. The conversion of this PSC bank into a therapeuticall
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Genomic changes at the chromosomal or single nucleotide leve
2018-10-20
Genomic changes at the chromosomal or single nucleotide level likely contribute to the variability of the pluripotent states and differentiation potential. These changes can be acquired and accumulated during cell culture because of suboptimal growth media, inappropriate extracellular matrices, and
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As METH impairs self renewal capacity we also explored the
2018-10-20
As METH impairs self-renewal capacity, we also explored the influence of METH in DG stem cell-fate division, a suitable method to characterize the phenotype of cells that are derived from the division of one DG stem cell. Indeed, a stem cell can undergo symmetric cell division towards self-renewal,
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Several lines of evidence suggest that mitochondrial insuffi
2018-10-20
Several lines of evidence suggest that mitochondrial insufficiency and failure to leave glycolysis (Warburg metabolism) are the fundamental limits to normal differentiation after FGF4 removal. These lines of evidence also pinpoint the two lineages at the labyrinthine surface as the highest energy- a
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We next aimed to test the differentiation capabilities of
2018-10-20
We next aimed to test the differentiation capabilities of the EZ spheres. We found that at any passage for all cell lines regardless of cryopreservation, EZ spheres can be placed in specific neural induction media for terminal neuronal or glial cell differentiation using protocols adapted from those
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