• GFP expressing Rhesus nhpESC lines Table

  2018-10-22

  

  GFP-expressing Rhesus nhpESC lines (Table 1) used in the aggregation chimera assay produced expanded mouse blastocysts with GFP-expressing nhpESC lines 2706 and 3006 associated with the blastocyst inner cell mass (ICM) cells. We typically combined mouse zona pellucida-free 2-to-8-cell stage embryos

• Previous experiments with mouse chimaeras and X inactivation

  2018-10-22

  

  Previous experiments with mouse chimaeras and X-inactivation mosaics (Collinson et al., 2002, 2004; Mort et al., 2009) suggested that the numbers of active LESC clones decline with age in wild-type mice and are reduced in Pax6+/− heterozygotes. Although this would imply that LESC function is deficie

• The immunophenotype of all three populations was comprehensi

  2018-10-22

  

  The immunophenotype of all three populations was comprehensively compared using a panel of 81 PE-conjugated tetracycline hcl (Supplementary Table 1). A heat map showed extensive congruence in the percentage of cells positive for a given CD epitope between the three populations, although hierarchical

• We aimed here to elucidate whether the effect

  2018-10-22

  

  We aimed here to elucidate whether the effect of taurine increasing proliferation occurs on stem cells as well as on neural progenitor cells. Neurospheres are formed by caspofungin of neural progenitor cells around one or several stem cells, but may also be formed by clusters of progenitor cells on

• To define the transcriptome of the ExE

  2018-10-22

  

  To define the transcriptome of the ExE lineage in the present study, we analyzed primitive endoderm and trophectoderm markers using qRT-PCR. Interestingly, we found that markers of the primitive endoderm, such as DDAH2, LAMB1, DAB2, AFP and NR2F1 (COUP-TF1), and markers of the trophectoderm, such as

• The advantages of CP could be even more profound for

  2018-10-22

  

  The advantages of CP could be even more profound for manufacturing cell therapies. In a typical cell therapy workflow, hPSCs are expanded and banked in a GMP facility before undergoing expensive and time-consuming tests to validate the cell bank. The conversion of this PSC bank into a therapeuticall

• Genomic changes at the chromosomal or single nucleotide leve

  2018-10-20

  

  Genomic changes at the chromosomal or single nucleotide level likely contribute to the variability of the pluripotent states and differentiation potential. These changes can be acquired and accumulated during cell culture because of suboptimal growth media, inappropriate extracellular matrices, and

• As METH impairs self renewal capacity we also explored the

  2018-10-20

  

  As METH impairs self-renewal capacity, we also explored the influence of METH in DG stem cell-fate division, a suitable method to characterize the phenotype of cells that are derived from the division of one DG stem cell. Indeed, a stem cell can undergo symmetric cell division towards self-renewal,

• Several lines of evidence suggest that mitochondrial insuffi

  2018-10-20

  

  Several lines of evidence suggest that mitochondrial insufficiency and failure to leave glycolysis (Warburg metabolism) are the fundamental limits to normal differentiation after FGF4 removal. These lines of evidence also pinpoint the two lineages at the labyrinthine surface as the highest energy- a

• We next aimed to test the differentiation capabilities of

  2018-10-20

  

  We next aimed to test the differentiation capabilities of the EZ spheres. We found that at any passage for all cell lines regardless of cryopreservation, EZ spheres can be placed in specific neural induction media for terminal neuronal or glial cell differentiation using protocols adapted from those

• br Acknowledgments This study was funded by a grant provided

  2018-10-20

  

  Acknowledgments This study was funded by a grant provided by the Royan Institute. We thank Mr. Ehsan Janzamin for his unlimited assistance in the Flow Cytometry Laboratory, Dr. Abass Piryaei and Leila Sattarian in the Histology Laboratory, Mr. Chehrazi for statistical analysis, and Miss Saloomeh

• The following is the supplementary data related

  2018-10-20

  

  The following is the supplementary data related to this article. Author contribution Acknowledgments Introduction Endothelial progenitor alzheimer\'s disease (EPCs) circulate in peripheral blood and migrate to a site for vascular formation and repair (Asahara et al., 1997). The pro-angiog

• br Introduction Stem cells play a crucial role in maintainin

  2018-10-20

  

  Introduction Stem wst-1 play a crucial role in maintaining tissue homeostasis. In this context, interplay between stem cells and their microenvironment, also known as the niche, is necessary for the maintenance of stem cell phenotypic state (Scadden, 2014). In general, distinct stem cell populat

• The karyotype of the MPSII iPSC line

  2018-10-20

  

  The karyotype of the MPSII-2.5 iPSC line was determined by Giemsa-banding, proving normal colorimetric assay 46, XY karyotype, without any detectable abnormalities (Fig. 1B). Materials and methods Acknowledgement We would like to thank the Department of Pediatrics, University of Pécs (Hungar

• br Results br Discussion The availability of Lgr as a

  2018-10-20

  

  Results Discussion The availability of Lgr5 as a specific marker for stem STF-62247 in the intestine and other tissues has allowed the unequivocal identification of intestinal stem cells, as well as molecular profiling (Muñoz et al., 2012), the establishment of culture methods (Sato et al.,

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